Oxazole yellow dye interactions with short DNA oligomers of homogeneous base composition and their hybrids.
Identifieur interne : 003988 ( Main/Exploration ); précédent : 003987; suivant : 003989Oxazole yellow dye interactions with short DNA oligomers of homogeneous base composition and their hybrids.
Auteurs : L D Simon [États-Unis] ; K H Abramo ; J K Sell ; L B McgownSource :
- Biospectroscopy [ 1075-4261 ] ; 1998.
Descripteurs français
- KwdFr :
- ADN simple brin (), ADN simple brin (métabolisme), Benzoxazoles (métabolisme), Colorants fluorescents (métabolisme), Composition en bases nucléiques, Conformation d'acide nucléique, Dichroïsme circulaire, Fixation compétitive, Polarisation de fluorescence, Poly A (), Poly A (métabolisme), Poly C (), Poly C (métabolisme), Poly DA-DT (), Poly DA-DT (métabolisme), Poly G (), Poly G (métabolisme), Poly T (), Poly T (métabolisme), Polydésoxyribonucléotides (), Polydésoxyribonucléotides (métabolisme), Quinoléines (métabolisme), Relation structure-activité, Spectrométrie de fluorescence, Spectrophotométrie UV, Séquences répétées d'acides nucléiques.
- MESH :
- métabolisme : ADN simple brin, Benzoxazoles, Colorants fluorescents, Poly A, Poly C, Poly DA-DT, Poly G, Poly T, Polydésoxyribonucléotides, Quinoléines.
- ADN simple brin, Composition en bases nucléiques, Conformation d'acide nucléique, Dichroïsme circulaire, Fixation compétitive, Polarisation de fluorescence, Poly A, Poly C, Poly DA-DT, Poly G, Poly T, Polydésoxyribonucléotides, Relation structure-activité, Spectrométrie de fluorescence, Spectrophotométrie UV, Séquences répétées d'acides nucléiques.
English descriptors
- KwdEn :
- Base Composition, Benzoxazoles (metabolism), Binding, Competitive, Circular Dichroism, DNA, Single-Stranded (chemistry), DNA, Single-Stranded (metabolism), Fluorescence Polarization, Fluorescent Dyes (metabolism), Nucleic Acid Conformation, Poly A (chemistry), Poly A (metabolism), Poly C (chemistry), Poly C (metabolism), Poly G (chemistry), Poly G (metabolism), Poly T (chemistry), Poly T (metabolism), Poly dA-dT (chemistry), Poly dA-dT (metabolism), Polydeoxyribonucleotides (chemistry), Polydeoxyribonucleotides (metabolism), Quinolines (metabolism), Repetitive Sequences, Nucleic Acid, Spectrometry, Fluorescence, Spectrophotometry, Ultraviolet, Structure-Activity Relationship.
- MESH :
- chemical , chemistry : DNA, Single-Stranded, Poly A, Poly C, Poly G, Poly T, Poly dA-dT, Polydeoxyribonucleotides.
- chemical , metabolism : Benzoxazoles, DNA, Single-Stranded, Fluorescent Dyes, Poly A, Poly C, Poly G, Poly T, Poly dA-dT, Polydeoxyribonucleotides, Quinolines.
- Base Composition, Binding, Competitive, Circular Dichroism, Fluorescence Polarization, Nucleic Acid Conformation, Repetitive Sequences, Nucleic Acid, Spectrometry, Fluorescence, Spectrophotometry, Ultraviolet, Structure-Activity Relationship.
Abstract
Interactions between short single-stranded DNA oligomers of homogeneous base composition and the fluorescent probes oxazole yellow (YO) and its homodimer YOYO are described. The oligomers included 15-mers and 30-mers of polydA, polydT, polydG, and polydC. Interactions between the dyes and DNA hybrids formed from complementary homogeneous strands of equal length were also investigated. No interactions were observed between the dyes and the monomeric monophosphate nucleosides A, G, T, or C. The dyes were found to interact much more strongly with the purine oligomers polydA and polydG than with the pyrimidine oligomers polydT and polydC. PolydA of both lengths has strong interactions with YOYO, whereas the polydG 30-mer interacts strongly with monomeric YO. The 15-mers of polydG and polydC of both lengths show little interaction with either dye. Interactions of the dyes with the polydA/polydT and polydG/polydC hybrids tend to be dominated by interactions with polydA and polydG, respectively. Although dye interactions generally were facilitated by hybridization, particularly for polydA/polydT, the interactions were similar to those with the single strands and different from those that have been observed in long double-stranded DNA.
DOI: 10.1002/(sici)1520-6343(1998)4:1<17::aid-bspy2>3.0.co;2-p
PubMed: 9547011
Affiliations:
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Le document en format XML
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J K" uniqKey="Sell J" first="J K" last="Sell">J K Sell</name></author><author><name sortKey="Mcgown, L B" sort="Mcgown, L B" uniqKey="Mcgown L" first="L B" last="Mcgown">L B Mcgown</name></author></analytic><series><title level="j">Biospectroscopy</title><idno type="ISSN">1075-4261</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Composition</term><term>Benzoxazoles (metabolism)</term><term>Binding, Competitive</term><term>Circular Dichroism</term><term>DNA, Single-Stranded (chemistry)</term><term>DNA, Single-Stranded (metabolism)</term><term>Fluorescence Polarization</term><term>Fluorescent Dyes (metabolism)</term><term>Nucleic Acid Conformation</term><term>Poly A (chemistry)</term><term>Poly A (metabolism)</term><term>Poly C (chemistry)</term><term>Poly C (metabolism)</term><term>Poly G (chemistry)</term><term>Poly G (metabolism)</term><term>Poly T (chemistry)</term><term>Poly T (metabolism)</term><term>Poly dA-dT (chemistry)</term><term>Poly dA-dT (metabolism)</term><term>Polydeoxyribonucleotides (chemistry)</term><term>Polydeoxyribonucleotides (metabolism)</term><term>Quinolines (metabolism)</term><term>Repetitive Sequences, Nucleic Acid</term><term>Spectrometry, Fluorescence</term><term>Spectrophotometry, Ultraviolet</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN simple brin ()</term><term>ADN simple brin (métabolisme)</term><term>Benzoxazoles (métabolisme)</term><term>Colorants fluorescents (métabolisme)</term><term>Composition en bases nucléiques</term><term>Conformation d'acide nucléique</term><term>Dichroïsme circulaire</term><term>Fixation compétitive</term><term>Polarisation de fluorescence</term><term>Poly A ()</term><term>Poly A (métabolisme)</term><term>Poly C ()</term><term>Poly C (métabolisme)</term><term>Poly DA-DT ()</term><term>Poly DA-DT (métabolisme)</term><term>Poly G ()</term><term>Poly G (métabolisme)</term><term>Poly T ()</term><term>Poly T (métabolisme)</term><term>Polydésoxyribonucléotides ()</term><term>Polydésoxyribonucléotides (métabolisme)</term><term>Quinoléines (métabolisme)</term><term>Relation structure-activité</term><term>Spectrométrie de fluorescence</term><term>Spectrophotométrie UV</term><term>Séquences répétées d'acides nucléiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Single-Stranded</term><term>Poly A</term><term>Poly C</term><term>Poly G</term><term>Poly T</term><term>Poly dA-dT</term><term>Polydeoxyribonucleotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Benzoxazoles</term><term>DNA, Single-Stranded</term><term>Fluorescent Dyes</term><term>Poly A</term><term>Poly C</term><term>Poly G</term><term>Poly T</term><term>Poly 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compétitive</term><term>Polarisation de fluorescence</term><term>Poly A</term><term>Poly C</term><term>Poly DA-DT</term><term>Poly G</term><term>Poly T</term><term>Polydésoxyribonucléotides</term><term>Relation structure-activité</term><term>Spectrométrie de fluorescence</term><term>Spectrophotométrie UV</term><term>Séquences répétées d'acides nucléiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Interactions between short single-stranded DNA oligomers of homogeneous base composition and the fluorescent probes oxazole yellow (YO) and its homodimer YOYO are described. The oligomers included 15-mers and 30-mers of polydA, polydT, polydG, and polydC. Interactions between the dyes and DNA hybrids formed from complementary homogeneous strands of equal length were also investigated. No interactions were observed between the dyes and the monomeric monophosphate nucleosides A, G, T, or C. The dyes were found to interact much more strongly with the purine oligomers polydA and polydG than with the pyrimidine oligomers polydT and polydC. PolydA of both lengths has strong interactions with YOYO, whereas the polydG 30-mer interacts strongly with monomeric YO. The 15-mers of polydG and polydC of both lengths show little interaction with either dye. Interactions of the dyes with the polydA/polydT and polydG/polydC hybrids tend to be dominated by interactions with polydA and polydG, respectively. Although dye interactions generally were facilitated by hybridization, particularly for polydA/polydT, the interactions were similar to those with the single strands and different from those that have been observed in long double-stranded DNA.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Abramo, K H" sort="Abramo, K H" uniqKey="Abramo K" first="K H" last="Abramo">K H Abramo</name><name sortKey="Mcgown, L B" sort="Mcgown, L B" uniqKey="Mcgown L" first="L B" last="Mcgown">L B Mcgown</name><name sortKey="Sell, J K" sort="Sell, J K" uniqKey="Sell J" first="J K" last="Sell">J K Sell</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Simon, L D" sort="Simon, L D" uniqKey="Simon L" first="L D" last="Simon">L D Simon</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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